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[Virus-like particles in yeast protoplasts. I. Electron microscopic demonstration]
Abstract:
In ultrathin sections and negatively stained isolates from protoplasts of a strain of Saccharomyces cerevisiae showing abnormal morphology and degeneration phenomena the presence of virus-like particles within the cells could be demonstrated. The isometric particles have diameters of about 50 nm, the electron dense centre is surrounded by distinct envelope. Protoplastic containing up to 30,000 particles could be found. It could be demonstrated that there exists a correlation between the amount of particles and the degree of degeneration of the cellular substructure. Protoplasts with high titres of virus-like particles obviously undergo lysis. Plaque formation on agar plates could not be seen. It is concluded that the phenomena observed can be compared with cytopathological effects caused by viruses in other cell systems.
Insights
Virus-like particles were found in degenerating Saccharomyces cerevisiae cells. High particle amounts correlated with cell damage and lysis, suggesting a viral cause for these yeast cell abnormalities.
Area of Science:
- Microbiology
- Yeast Genetics
- Cell Biology
Background:
- Saccharomyces cerevisiae is a model organism in biological research.
- Abnormal cell morphology and degeneration phenomena in yeast can impact experimental outcomes.
- Understanding the causes of cellular damage is crucial for yeast biology.
Purpose of the Study:
- To investigate the presence and characteristics of virus-like particles in degenerating Saccharomyces cerevisiae.
- To determine the correlation between virus-like particles and cellular degeneration.
- To assess the potential role of these particles in yeast cell pathology.
Main Methods:
- Ultrathin sectioning of yeast protoplasts.
- Negative staining of isolated particles.
- Electron microscopy for particle visualization and characterization.
- Correlation analysis between particle load and cellular degeneration.
Main Results:
- Virus-like particles, approximately 50 nm in diameter with an electron-dense core and envelope, were identified within Saccharomyces cerevisiae protoplasts.
- Cells contained up to 30,000 particles.
- A positive correlation was observed between the quantity of virus-like particles and the extent of cellular substructure degeneration.
- Protoplasts with high particle titers exhibited lysis.
- No plaque formation was observed on agar plates.
Conclusions:
- The observed cytopathological effects in Saccharomyces cerevisiae are consistent with viral infection.
- Virus-like particles are strongly implicated as the causative agents of degeneration and lysis in this yeast strain.
- Further research may be needed to characterize the specific virus and its replication cycle.