Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence analysis of MAP2 function in living cells

J Ferralli1, T Doll, A Matus

  • 1Friedrich Miescher Institute, Basel, Switzerland.

Journal of Cell Science
|November 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply to Comment on 'Canine scent detection for the diagnosis of lung cancer in a screening-like situation'.

Journal of breath research·2017
Same author

Tetrodotoxin-sensitive α-subunits of voltage-gated sodium channels are relevant for inhibition of cardiac sodium currents by local anesthetics.

Naunyn-Schmiedeberg's archives of pharmacology·2016
Same author

Dispersed Hydrogel Actuator for Modiolar Hugging Cochlear Implant Electrode Arrays.

IEEE transactions on bio-medical engineering·2016
Same author

Methadone is a local anaesthetic-like inhibitor of neuronal Na+ channels and blocks excitability of mouse peripheral nerves.

British journal of anaesthesia·2014
Same author

Opposite effect of fluticasone and salmeterol on fibronectin and tenascin-C expression in primary human lung fibroblasts.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2009
Same author

[Erythema necroticans migrans in non-glucagon-secreting pancreas tumor].

Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete·2007
Same journal

Potential role of myeloid bodies in protection against photo-oxidative damage of the retinal pigment epithelium.

Journal of cell science·2026
Same journal

From the FAZ to the TAC: Widespread cytoskeletal engagement of T. brucei KMP11.

Journal of cell science·2026
Same journal

Branched actin segregates endocytic cargo to control sorting and fission.

Journal of cell science·2026
Same journal

FAM122A inhibition of PP2A-B55 through a bipartite binding mechanism.

Journal of cell science·2026
Same journal

Disentangling the response to lysosomal damage.

Journal of cell science·2026
Same journal

The force, form and function of the nucleus.

Journal of cell science·2026
See all related articles

Microtubule-associated protein 2 (MAP2) binding to microtubules involves multiple weak sites, not just repeats. This interaction stabilizes microtubules, promoting process outgrowth in non-neuronal cells.

Area of Science:

  • Cell Biology
  • Neuroscience
  • Protein Biochemistry

Background:

  • Microtubule-associated protein 2 (MAP2) is crucial for neuronal structure.
  • MAP2 binds microtubules via carboxyl-terminal repeats, influencing microtubule stability.

Purpose of the Study:

  • To identify specific MAP2 sequence elements responsible for microtubule binding and stabilization.
  • To investigate the relationship between MAP2 binding strength and its effects on microtubule bundling and process outgrowth.

Main Methods:

  • Construction and transfection of MAP2c deletion mutants into non-neuronal cells.
  • Analysis of mutant MAP2 binding affinity to microtubules.
  • Assessment of microtubule bundling and process outgrowth support.

Related Experiment Videos

Main Results:

  • Microtubule binding strength increased with the number of 31-amino acid repeats.
  • The repeat domain alone was insufficient; adjacent amino-terminal and carboxyl-terminal sequences were required for binding.
  • Binding strength correlated with microtubule bundling and process outgrowth support.

Conclusions:

  • MAP2-microtubule interaction is mediated by multiple additive weak binding sites, including repeats and flanking sequences.
  • Microtubule bundling and stiffening by MAP2 are direct consequences of strong microtubule stabilization.