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Plasmodesmata of maize root tips: structure and composition

A Turner1, B Wells, K Roberts

  • 1Department of Cell Biology, John Innes Centre, Norwich, UK.

Journal of Cell Science
|December 1, 1994
PubMed
Summary
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Researchers developed a method to isolate maize cell walls with intact plasmodesmata. Biochemical treatments revealed that while some parts can be removed, the core plasmodesmata structure remains, aiding molecular component characterization.

Area of Science:

  • Plant Biology
  • Cell Biology
  • Biochemistry

Background:

  • Plasmodesmata are crucial for intercellular communication in plants.
  • Understanding plasmodesmata composition is key to deciphering plant cell-to-cell transport.
  • Previous methods lacked the resolution to dissect plasmodesmata components within the cell wall.

Purpose of the Study:

  • To develop a procedure for isolating maize cell walls containing intact plasmodesmata.
  • To biochemically probe the composition and structure of plasmodesmata and their associated components.
  • To identify potential molecular components of plasmodesmata for further characterization.

Main Methods:

  • Isolation of clean maize cell wall preparations.
  • Transmission electron microscopy with negative staining and rotary shadowing.

Related Experiment Videos

  • Biochemical treatments including light and heavy proteolysis, urea extraction, and detergent extraction.

    • Main Results:

    • Light proteolysis removed superficial material but not the plasmodesmal core.
    • Heavy proteolysis and urea extraction could remove the entire plasmodesma, including the collar.
    • Callose was localized around plasmodesmata but not in the collar; membrane components were extracted by detergents, releasing potential plasmodesmal proteins.

    Conclusions:

    • Maize plasmodesmata can be biochemically dissected.
    • The study provides a strategy for characterizing individual molecular components of plasmodesmata.
    • The collar structure's non-proteinaceous nature and callose localization were elucidated.