Isolation of cytoskeletal proteins from Drosophila

In this summary of methods, we have attempted to present a series of protocols relevant to the study of proteins that associate with each other inside cells. These techniques have found their main application, in our hands, to cytoskeletal structures, but the utility of these techniques is limited to this application. As we learn more about the physiology and organization of eukaryotic cells, information from a variety of studies and systems indicates that many processes occur in a highly spatially organized manner in cells, using multiprotein complexes. The techniques described here will find application to the study of how organization and associations between proteins contribute to many of cellular processes.