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Adhesion molecules in the nickel allergic reaction
S Silvennoinen-Kassinen1, O Vainio, J Karvonen
1Department of Medical Microbiology, University of Turku, Finland.
International Archives of Allergy and Immunology
|April 1, 1995
Summary
Nickel allergy involves changes in immune cell adhesion molecules. Nickel exposure upregulates specific adhesion molecules on CD4+ T cells while downregulating them on CD8+ T cells, particularly evident in vitro.
Area of Science:
- Immunology
- Dermatology
Background:
- Nickel is a primary cause of allergic contact dermatitis.
- Understanding nickel-induced immune responses is crucial for managing this condition.
Purpose of the Study:
- To investigate alterations in adhesion molecule expression on mononuclear cells during nickel stimulation.
- To compare in vivo and in vitro nickel-induced immune responses.
Main Methods:
- Utilized nickel-induced lymphocyte cultures (in vitro) and skin biopsies from nickel reactions (in vivo).
- Employed monoclonal antibodies (Mabs) and flow cytometry for in vitro analysis.
- Used Mabs and immunohistochemistry for in vivo analysis of mononuclear cells.
Main Results:
- In vitro, nickel stimulation led to differential expression of adhesion molecules on lymphoblasts.
- CD4+ T cells showed increased adhesion molecule expression, while CD8+ T cells showed decreased expression.
- In vivo results largely mirrored in vitro findings, with some differences in molecule detection.
Conclusions:
- Nickel-induced allergic reactions modulate adhesion molecule expression, favoring certain molecules on CD4+ cells and reducing them on CD8+ cells.
- In vitro methods demonstrated higher sensitivity in detecting these adhesion molecule changes.