[Amplification, cloning and sequencing of beta nerve growth factor gene in the Chinese population]

Genomic DNA was extracted from white blood cells. Using the genomic DNA as template, the gene encoding nerve growth factor (beta-NGF) in the Chinese population was amplified using polymerase chain reaction (PCR). The beta-NGF gene obtained by PCR was then cloned into plasmid pUC 12 which was digested with SmaI. After transformation, screening and identification, 8 clones containing the beta-NGF gene were analyzed. Nucleotide sequence of the NGF was determined by dideoxy-nucleotide chain-termination method. The sequencing result showed that there are 6 differences between the beta-NGF sequence of the Chinese population and previous reported beta-NGF sequence.