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Site-specific 15N-labelling of oligonucleotides for NMR: the trp operator and its interaction with the trp repressor
V Ramesh1, Y Z Xu, G C Roberts
1Department of Biochemistry and Biological NMR Centre, University of Leicester, UK.
FEBS Letters
|April 17, 1995
Abstract:
A convenient and economical method is described for the site-specific 15N-labelling of the 4-amino group of individual cytidine residues in oligonucleotides. This is applied to a 20 base-pair oligonucleotide corresponding to the trp operator; this oligonucleotide and its complex with the E. coli trp repressor are studied by NMR, using 1H-15N HMQC and 15N-edited 1H-1H NOESY experiments.