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Sequence and genomic organization of a rabbit hemorrhagic disease virus isolated from a wild rabbit
D Rasschaert1, S Huguet, M F Madelaine
1Unité de Virologie et d'Immunologie Moléculaires, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
Abstract:
Rabbit hemorrhagic disease virus (RHDV) is a member of the caliciviridae family. The nucleotidic sequence of a full-length cDNA of one RHDV isolate (RHDV-SD) is reported. The genome is 7437 bases long and includes two ORFs, ORF1 (7034 b) and ORF2 (353 b), coding for the polyprotein and the Vp12, respectively. The coding sequence for the second structural protein (the capsid protein, Vp60) is located at the 3' end of ORF1. Comparison of RHDV-SD with the German RHDV isolated revealed 470 nucleotide substitutions (96% homology). The deduced amino acid sequences of the two isolates are closely related (98% identity), and no hypervariable region could be identified either in the structural or nonstructural proteins.