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[Study of intestinal microflora in children with acute and persistent diarrhea]
Y Garcia Cristia1, T Fragoso Arbelo, M Valdes-Dapena Vivanco
1Instituto de Ciencias Médicas de la Habana.
Insights
Altered enteric microflora, including bacterial overgrowth, is a significant risk factor for persistent diarrhea in infants. Microbiological analysis of duodenal content revealed both quantitative and qualitative changes in gut flora.
Area of Science:
- Gastroenterology
- Microbiology
- Pediatrics
Background:
- Persistent diarrhea in infants often has multifactorial or unidentified causes.
- The role of enteric microflora alterations as a risk factor for diarrhea duration requires investigation.
Purpose of the Study:
- To investigate if changes in enteric microflora contribute to the persistence of diarrhea in infants.
- To correlate duodenal microflora composition with clinical outcomes and nutritional status.
Main Methods:
- Studied 30 infants with acute diarrhea and 30 with persistent diarrhea.
- Collected duodenal content samples using a double-lumen tube for microbiological analysis.
- Examined for enteric microorganisms, anaerobes, and Candida, correlating findings with nutritional status and stool cultures.
Main Results:
- Identified quantitative and qualitative alterations in duodenal flora in both acute and persistent diarrhea groups.
- Bacterial overgrowth in the duodenum was found to be a significant factor in persistent diarrhea.
- Duodenal microflora analysis provided insights into the pathogenesis of prolonged diarrhea.
Conclusions:
- Alterations in duodenal microflora, including bacterial overgrowth, play a crucial role in the persistence of diarrhea in infants.
- Microbiological assessment of the gut flora is important for understanding and managing persistent diarrhea.
- Further research into enteric microflora's impact on infant diarrhea is warranted.
Abstract:
The etiology and pathogenesis of persistent diarrhoea is usually multifactorial and sometimes can not be identified. It is necessary to define if an alteration of the enteric microflora is a risk factor that influence the duration of the diarrhoea. 30 infants with acute diarrhoea and 30 with persistent diarrhoea were studied. A sample of duodenal content was taken by a double-lumen tube and processed microbiologically in search for enteric microorganisms, anaerobic and Candida. These results were correlated with the nutritional status, the previous use of Metronidazole and the results of the stool culture. The presence of bacterial overgrowth and the identification of the duodenal microflora is an important factor for the persistence of the diarrhoea. There was a quantitative and qualitative alteration of the duodenal flora in both groups of patients.