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Related Experiment Videos

Actin-based bacterial motility

P Cossart1

  • 1Unité des Interactions Bactéries-Cellules, Institut Pasteur, Paris, France.

Current Opinion in Cell Biology
|February 1, 1995
PubMed
Summary
This summary is machine-generated.

Bacterial pathogens like Listeria monocytogenes use actin-based motility for movement within host cells. Key bacterial proteins, ActA and IcsA, drive this process, with recent discoveries revealing their phosphorylation and host cell interactions.

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Area of Science:

  • Microbiology
  • Cell Biology
  • Biochemistry

Background:

  • Bacterial pathogens such as Listeria monocytogenes exhibit actin-based motility within host cells.
  • This movement is propelled by the host cell's actin cytoskeleton, assembled at the bacterial pole.
  • The bacterial proteins ActA (in Listeria) and IcsA (in Shigella) are essential for mediating this motility.

Purpose of the Study:

  • To summarize recent key discoveries in bacterial actin-based motility.
  • To highlight the roles of bacterial surface proteins ActA/IcsA.
  • To underscore the importance of host-pathogen interactions in bacterial dissemination.

Main Methods:

  • Review of recent findings on bacterial motility mechanisms.
  • Analysis of protein localization (ActA/IcsA) on bacterial surfaces.

Related Experiment Videos

  • Investigation of host cell protein involvement (e.g., profilin).
  • Examination of ActA expression in transfected eukaryotic cells.
  • Utilizing a cell-free system to study Listeria motility.

    • Main Results:

    • ActA and IcsA proteins are localized to the bacterial pole.
    • Bacterial ActA undergoes phosphorylation within infected host cells.
    • Host cell protein profilin plays a role in actin assembly.
    • Expression of ActA in eukaryotic cells can induce actin polymerization and cell movement.
    • A cell-free system successfully reconstitutes bacterial actin-based motility.

    Conclusions:

    • Actin-based motility is a critical mechanism for bacterial invasion and spread.
    • The interaction between bacterial proteins (ActA/IcsA) and host cell machinery is complex.
    • Recent advances, including cell-free systems, facilitate deeper understanding of this phenomenon.