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Cloning and mapping of murine Nfe2l1
J McKie1, K Johnstone, M G Mattei
1Molecular Medicine Unit, Institute of Child Health, London, United Kingdom.
Genomics
|February 10, 1995
Summary
Researchers identified the mouse NFE2L1 gene, crucial for human health, finding 97% similarity to its human counterpart. This discovery aids understanding of gene function and evolution in mammals.
Area of Science:
- Molecular Biology
- Genetics
- Comparative Genomics
Background:
- The human NFE2L1 gene encodes a basic leucine-zipper transcription factor involved in cellular processes.
- Understanding homologous genes across species aids in elucidating conserved biological functions and evolutionary pathways.
Purpose of the Study:
- To isolate and characterize the murine homologue of the human NFE2L1 gene.
- To investigate the sequence similarity and evolutionary relationships of murine Nfe2l1 with known proteins.
Main Methods:
- Screening of an early embryo cDNA library to isolate the murine Nfe2l1 gene.
- Deduction of amino acid sequence and comparison with human NFE2L1 and other related proteins (NF-E2 p45, Drosophila CNC).
- Chromosomal mapping of the murine Nfe2l1 gene.
Main Results:
- Successful isolation of the murine Nfe2l1 gene.
- The deduced amino acid sequence of murine Nfe2l1 exhibits 97% identity to human NFE2L1.
- Significant sequence similarity was observed with NF-E2 p45 subunit and Drosophila CNC protein, indicating conserved domains.
- Murine Nfe2l1 was mapped to chromosome 11DE, with additional similar sequences located at 7D1-7F1 and 2E4-2G.
Conclusions:
- The high sequence identity suggests a conserved function between human and murine NFE2L1.
- The findings support the evolutionary conservation of the NFE2L1 gene family across species.
- Chromosomal mapping provides a basis for further genetic studies and understanding gene regulation in mice.