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Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

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STM and AFM in biotechnology

L Haggerty¹, A M Lenhoff

¹Department of Chemical Engineering, University of Delaware, Newark 19716.

Biotechnology Progress

|January 1, 1993

Summary

Scanning tunneling microscopy (STM) and atomic force microscopy (AFM) offer atomic-scale imaging for biological materials. This review details their application in biotechnology, focusing on DNA and protein studies.

Area of Science:

Surface science
Biotechnology
Nanotechnology

Background:

Scanning tunneling microscopy (STM) and atomic force microscopy (AFM) provide atomic-scale imaging of surfaces.
These techniques can be applied to biological materials by depositing them on suitable substrates.
Previous studies have imaged biological samples with varying resolution.

Purpose of the Study:

To review the principles of STM and AFM.
To discuss practical implementation of STM and AFM for biotechnology.
To focus on the study of biological macromolecules like DNA and proteins.

Main Methods:

Summarizing the principles of STM and AFM.
Discussing sample preparation, substrates, imaging media, tips, and operating conditions.

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Reviewing existing literature on STM/AFM applications in biotechnology.

Main Results:

STM and AFM yield high-resolution images of biological surfaces.
The review covers essential parameters for successful imaging of biomolecules.
Practical considerations for applying these techniques to DNA and proteins are detailed.

Conclusions:

STM and AFM are powerful tools for atomic-scale analysis of biological macromolecules.
The review provides a practical guide for researchers in biotechnology.
Future applications of STM and AFM in biotechnology show significant promise.