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Separation of specific immunoglobulin. 1. Desalination using a membrane system
G Tishchenko1, M Bleha, J Skvor
1Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic.
Bioseparation
|February 1, 1995
Summary
This study presents a novel, rapid method for desalting immunoglobulin fractions from mouse ascitic fluid using membrane dialysis. The technique significantly reduces processing time from five days to a few hours, achieving high purity and yield.
Area of Science:
- Biochemistry
- Biotechnology
- Separation Science
Background:
- Immunoglobulin fractions are often purified using ammonium sulfate precipitation.
- Traditional desalting methods for these fractions are time-consuming.
- Efficient separation of immunoglobulins from salts is crucial for downstream applications.
Purpose of the Study:
- To investigate a non-traditional dialysis method for separating ammonium sulfate from immunoglobulin fractions.
- To optimize parameters for rapid and high-purity immunoglobulin desalting.
- To reduce the processing time for immunoglobulin purification.
Main Methods:
- Utilized spiral membrane modules with anion exchange and microfiltration membranes.
- Employed a counterpressure-driven dialysis technique to enhance salt and low-molecular-weight component diffusion.
- Varied flow rate, counterpressure, and microfiltration membrane pore size.
Main Results:
- Achieved high purity and yield of the specific immunoglobulin fraction (pI 6.7-6.8, MW 180,000).
- Reduced desalting time from 5 days to a few hours.
- Demonstrated that flow rate, counterpressure, and pore size significantly impact separation efficiency.
Conclusions:
- The developed membrane dialysis method offers a rapid and efficient alternative for desalting immunoglobulin fractions.
- This technique is suitable for large-scale purification, significantly improving workflow efficiency.
- The process yields highly pure immunoglobulin, suitable for various research and therapeutic applications.