Related Experiment Videos
Developmental potential of microbiopsied murine blastocysts
1Fertility Institute, Methodist Hospital of Indiana, Indianapolis.
Obstetrics and Gynecology
|January 1, 1995
Summary
Blastocyst biopsy and embryo transfer in mice did not significantly affect the rate of live-born pups. This study indicates that the biopsy procedure does not harm murine blastocyst developmental potential.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Genetics
Background:
- Blastocyst biopsy is a technique used in assisted reproductive technologies.
- Evaluating the impact of biopsy on embryo development is crucial for its clinical application.
Purpose of the Study:
- To assess the live-birth rate in mice following blastocyst biopsy and embryo transfer.
- To compare outcomes between biopsied and non-biopsied control embryos.
Main Methods:
- Embryos from ICR Swiss albino and C57B1/6 mice were collected on day 4.
- Hatching trophoblast was excised from experimental embryos using micromanipulation.
- Biopsied (206) and control (206) embryos were transferred to pseudopregnant recipients.
Main Results:
- The percentage of live-born pups from control embryos was 27.7%.
- The percentage of live-born pups from biopsied embryos was 34.5%.
- No statistically significant difference was observed between the two groups (P > .1).
Conclusions:
- The developmental potential of murine blastocysts is not adversely affected by the biopsy procedure.
- These findings support the safety of blastocyst biopsy for embryo transfer.