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Developmental patterns of histamine-like immunoreactivity in the mouse
1Department of Anatomy, University of Helsinki, Finland.
Summary
Histamine (HA) appears in various mouse embryonic cells, including mast cells, endocrine cells, and neurons. By adulthood, HA is mainly found in mast cells, oxyntic cells, and brain neurons, indicating developmental regulation.
Area of Science:
- Developmental Biology
- Neuroscience
- Immunology
Background:
- Histamine (HA) plays crucial roles in physiological processes.
- Understanding histamine's developmental expression is key to its functions.
- Previous studies have not fully mapped HA distribution during mouse development.
Purpose of the Study:
- To investigate the appearance and distribution of histamine (HA) during mouse embryogenesis, neonatal period, and adulthood.
- To identify specific cell types and organs expressing HA throughout development.
Main Methods:
- Utilized a specific rabbit HA antiserum for indirect immunofluorescence.
- Examined HA expression across embryonic day 13 (E13) through adulthood in mice.
- Analyzed HA immunoreactivity in various tissues, including gastrointestinal tract, liver, spleen, nervous system, kidney, and pancreas.
Main Results:
- HA first detected at E13 in mast cells and splenic primordium.
- From E15, HA-immunoreactive (HA-ir) cells found in epithelial endocrine cells, peripheral nervous system (PNS) neurons and fibers, kidney tubules, and pancreatic islets.
- Mast cell density increased with age; adult HA expression limited to mast cells, oxyntic cells, and tuberomamillary nucleus neurons.
Conclusions:
- Histamine immunoreactivity is widely distributed in epithelial, neuronal, and mast cells during mouse embryogenesis.
- HA expression patterns change significantly from embryonic stages to adulthood.
- This study provides a comprehensive map of HA distribution during mouse development.