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The deduced Vibrio cholerae RecA amino-acid sequence
R L Margraf1, A I Roca, M M Cox
1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1569.
Gene
|January 11, 1995
Summary
The recA gene sequence from Vibrio cholerae was determined. Its protein product is similar to other RecA proteins but differs from a previously published sequence.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- The recA gene is crucial for DNA repair and recombination in bacteria.
- Vibrio cholerae is a significant human pathogen.
- Previous reports on the Vibrio cholerae recA gene sequence exist.
Purpose of the Study:
- To determine the nucleotide sequence of the recA gene in Vibrio cholerae.
- To compare the deduced amino acid sequence with known RecA proteins.
- To resolve discrepancies with previously reported sequences.
Main Methods:
- DNA sequencing of the Vibrio cholerae recA gene.
- Bioinformatic analysis of nucleotide and amino acid sequences.
Main Results:
- The complete nucleotide sequence of the Vibrio cholerae recA gene was determined.
- The deduced amino acid sequence shows high similarity to other bacterial RecA proteins.
- A significant disagreement was found when compared to a previously published Vibrio cholerae RecA amino acid sequence.
Conclusions:
- This study provides an accurate sequence for the Vibrio cholerae recA gene.
- The findings highlight potential errors in prior sequence data.
- Accurate RecA sequences are vital for understanding DNA repair mechanisms in Vibrio cholerae.