Polymeric reagents for derivatizations in micellar electrokinetic chromatography

Area of Science:

• Analytical Chemistry
• Separation Science
• Chromatography

Background:

• Micellar electrokinetic chromatography (MEKC) is a powerful separation technique.
• Analysis of amine drugs in biological samples often requires complex sample preparation.
• Development of efficient derivatization reagents is crucial for improving MEKC analysis.

Purpose of the Study:

• To develop and characterize a polymer-immobilized o-nitrobenzophenone reagent for amine drug analysis.
• To evaluate the reagent's performance in micellar electrokinetic chromatography (MEKC).
• To validate the method for quantifying adamantanamine in biological matrices.

Main Methods:

• Preparation of a polymer-immobilized o-nitrobenzophenone reagent.
• On-instrument derivatization of amine drugs directly in the sample vial.
• Direct injection of the derivatized mixture onto the MEKC system.
• Validation of the method using propylamine, n-alkyl amines, amino acids, and adamantanamine in urine and plasma.

Main Results:

• The polymer-immobilized reagent demonstrated effective derivatization of various amines.
• The method allowed direct injection, minimizing sample preparation steps.
• Precisions and accuracies were below 6.0% for urine and 10% for plasma samples for adamantanamine analysis.

Conclusions:

• A polymer-immobilized o-nitrobenzophenone reagent is suitable for MEKC analysis of amine drugs.
• The developed method offers a simplified, direct injection technique for biofluid analysis.
• This approach enhances efficiency and accuracy in drug quantification within biological samples.