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[The persistence of Shigella sonnei in epithelial tissue in Serény's model]
Abstract:
The duration of persistence of S. sonnei in the tissues of a guinea pig eye (Serény's model) has been studied. Bacteriological and histological investigations made with the use of fluorescent antibody techniques have revealed that S. sonnei can be preserved in macrophages (histiocytes) and intercellular space of the eye epithelium for a long time (up to 277 days). The injection of ACTH or Shigella vaccine into the animals may provoke the exacerbation of the infectious process and the release of the infective agent.
Insights
Shigella sonnei can persist in guinea pig eyes for up to 277 days, primarily within macrophages. Immune stimulation can reactivate the infection, releasing the bacteria.
Area of Science:
- Ophthalmology
- Microbiology
- Immunology
Background:
- Shigella sonnei is a pathogen causing bacillary dysentery.
- Ocular infections with S. sonnei are rare but can be severe.
- The Serény's model in guinea pigs is used to study Shigella infections.
Purpose of the Study:
- To determine the duration of S. sonnei persistence in the guinea pig eye.
- To investigate the cellular location and potential for reactivation of S. sonnei within ocular tissues.
Main Methods:
- Bacteriological and histological investigations were performed.
- Fluorescent antibody techniques were utilized for detection.
- The Serény's model (guinea pig eye) was employed.
Main Results:
- S. sonnei persisted in guinea pig eye tissues for up to 277 days.
- The bacteria were found within macrophages (histiocytes) and the intercellular space of the eye epithelium.
- Administration of ACTH or Shigella vaccine led to exacerbation and release of the infective agent.
Conclusions:
- S. sonnei establishes long-term persistence in ocular tissues.
- Macrophages serve as a reservoir for S. sonnei.
- Immune modulation can trigger bacterial reactivation and dissemination.