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Updated: Oct 22, 2026
Non-invasive Imaging and Analysis of Cerebral Ischemia in Living Rats Using Positron Emission Tomography with 18F-FDG
Published on: December 28, 2014
Changes in metabolites and tissue water status after focal ischemia in cat brain assessed with localized proton MR
A van der Toorn1, H B Verheul, J W Berkelbach van der Sprenkel
1Department of in vivo NMR, Bijvoet Centre for Biomolecular Research, Utrecht University, The Netherlands.
Abstract:
Localized proton spectroscopy was used to monitor changes in metabolism and the biophysical status of tissue water in cat brain induced by occlusion of the middle cerebral artery. Changes in the intensity of N-acetyl-aspartate (NAA), total creatine (tCr), and lactate (Lac) signals in localized volumes of interest in the ischemic hemisphere were quantified relative to the preischemic signal. Changes in the apparent diffusion coefficient (ADC), T1- and T2-relaxation times of water in those volumes were also quantified. Lactate was shown to increase rapidly in the first 0.5-2.0 h of ischemia and stabilized afterwards. The ADC of water started to decrease from 0.64 x 10(-9) m2/s to 0.54 x 10(-9) m2/s in the first minutes following occlusion, as was shown in two cases where ADC was measured with high temporal resolution, and stabilized after approximately 3 h at 0.38 x 10(-9) m2/s (n = 6). NAA and tCr decreased by 35% (P < 0.0001) and 30% (P < 0.005), respectively, in the first 8 h of ischemia in comparison with the preischemic control levels. T1 and T2 gradually increased with 0.3 s (P < 0.0001) and 5.2 ms (P < 0.0001), respectively, during the same time span.
