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New application of silane coupling agents for covalently binding antibodies to glass and cellulose solid supports
N M Pope1, D L Kulcinski, A Hardwick
1Baxter Healthcare Corporation, Biotech Group-Immunotherapy Division, Santa Ana, California 92705.
Abstract:
Bifunctional silane reagents (3-iodopropyl)trimethoxysilane (1), (gamma-glycidoxypropyl)trimethoxysilane (2), and [1-(trimethoxysilyl)-2-(m- (or p-)chloromethyl)phenyl]ethane (3) were used to covalently link goat anti-mouse (GAM) antibodies (Ab) to glass microbeads and cuprammonium rayon hollow-fiber dialyzers. An average of 0.79 and 0.83 microgram of GAM Ab/cm2 was immobilized on the hollow-fiber dialyzers and the glass beads, respectively. The antibodies immobilized on glass microbeads or on hollow-fiber dialyzers were then used to selectively deplete CD34+ cells or CD4+ peripheral blood mononuclear cells (PBMC), respectively. Glass microbeads depleted 80% CD34+ cells with good selectivity, and the hollow-fiber dialyzers depleted an average of 81% CD4+ PBMC.