Anatrecon: a user guide to software package for computer-aided three-dimensional reconstructions

"Anatrecon" is an original Czech software package. It enables one to make easy three-dimensional reconstructions from serial sections of cells and tissues. It offers very acceptable information on the three-dimensional structure and especially on mutual relationships of biological objects in such cases when other methodical approaches, e.g., scanning electron microscopy or laser scanning confocal light microscopy, cannot be applied. The program runs on a standard IBM compatible personal computer supplemented with a digitizing tablet. The author describes the program in detail and presents examples of reconstructions of dendritic spine and nucleus of nerve cell.