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Updated: Aug 10, 2026

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Published on: February 6, 2018
Localization of basic and acidic argyrophilic proteins in nuclear structures of elongating mouse spermatids
1Institute of Histology and Embryology, 2nd Medical Faculty of Charles University, Praha, Czech Republic.
Abstract:
Different cytochemical techniques at the electron microscopic level were used to investigate the presence of basic and acidic proteins during spermiogenesis in mice. Basic lysine rich proteins were demonstrated with an ethanolic solution of phosphotungstic acid, acidic agryrophilic proteins using two silver staining procedures applied en bloc and on the ultrathin lowicryl sections. Both types of proteins had very similar localization in the nucleoplasm and identical distribution within the postacrosomal dense lamina on the surface of late spermatid heads. The specificity of reactions is briefly discussed.
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