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Bladder irrigation specimens assayed by fluorescence in situ hybridization to interphase nuclei
L L Wheeless1, J E Reeder, R Han
1Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, New York 14642.
Cytometry
|December 1, 1994
Summary
Bladder irrigation specimens can detect abnormal chromosome changes, like chromosome 9 loss, even when no bladder cancer is clinically evident. This method aids in monitoring patients for recurrent tumors.
Area of Science:
- Urothelial biology
- Cancer genetics
- Molecular diagnostics
Background:
- Bladder irrigation specimens offer a practical method for longitudinal monitoring of the bladder urothelium.
- Recurrent bladder tumors pose a significant clinical challenge, necessitating reliable monitoring methods.
Purpose of the Study:
- To investigate the utility of bladder irrigation specimens for fluorescence in situ hybridization (FISH) analysis in patients monitored for recurrent bladder tumors.
- To correlate FISH findings with DNA cytometry data to identify chromosomal aberrations associated with bladder cancer.
Main Methods:
- Cross-sectional FISH analyses were performed on bladder irrigation specimens from 76 patients.
- FISH probes targeted centromeric satellite sequences for chromosomes 1, 7, 9, 11, 15, and 17.
- DNA cytometry data was correlated with FISH results.
Main Results:
- Aberrations in chromosomes 1, 7, 11, and 17 were primarily observed in patients with aneuploid tumors.
- Monosomy of chromosome 9 was detected in 39% of diploid and 31% of hyperdiploid specimens.
- Significantly, 24% of patients with no clinical evidence of disease showed monosomy of chromosome 9, suggesting persistent abnormal cells.
Conclusions:
- Bladder irrigation specimens are a viable source for FISH analysis in monitoring bladder cancer.
- Chromosome 9 abnormalities, particularly monosomy, are prevalent in patients with a history of bladder cancer, even without current clinical disease.
- The study highlights the potential of FISH on bladder irrigation samples for early detection and monitoring of bladder tumor recurrence.