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Related Experiment Videos

Display and correction of flow cytometry time-dependent fluorescence changes

L Kusuda1, M R Melamed

  • 1Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York.

Cytometry
|December 1, 1994
PubMed
Summary

Flow cytometry measurements can vary due to staining and flow rate changes. This study presents a method to display and correct these time-dependent variations using chronological data analysis.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Analytical Chemistry

Background:

  • Flow cytometry is a powerful technique for analyzing cellular attributes based on fluorescent marker labeling.
  • Variations in staining intensity and sample stream flow can introduce time-dependent errors during data acquisition.
  • These variations can compromise the accuracy and resolution of flow cytometry measurements.

Purpose of the Study:

  • To introduce a novel method for visualizing and correcting time-dependent variations in flow cytometry data.
  • To improve the reliability and accuracy of cellular attribute measurements obtained by flow cytometry.
  • To address the impact of staining and flow rate fluctuations on data quality.

Main Methods:

  • Analysis of list mode data in chronological order.

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  • Development of a display method to visualize time-dependent changes.
  • Implementation of a correction strategy for identified variations.
  • Utilizing chronological data to identify and adjust for fluctuations.

    • Main Results:

    • Successful display of time-dependent variations in flow cytometry data.
    • Demonstration of effective correction for staining and flow rate induced changes.
    • Improved resolution and accuracy of cellular attribute measurements.
    • Validation of the chronological data analysis approach.

    Conclusions:

    • The described method effectively addresses time-dependent variations in flow cytometry.
    • Accurate cellular attribute measurement is achievable by correcting for data acquisition inconsistencies.
    • This approach enhances the robustness and reliability of flow cytometry analysis.