Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

G1 control in mammalian cells

S I Reed1, E Bailly, V Dulic

  • 1Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037.

Journal of Cell Science. Supplement
|January 1, 1994
PubMed
Summary

Agents blocking the G1/S cell cycle transition increase levels of cyclin-dependent kinase (Cdk) inhibitors. These inhibitors bind Cdks, halting cell cycle progression, with p21Waf1/Cip1 linked to DNA damage and p27Kip1 to antiproliferative conditions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Candida nivariensis: Identification strategy in mycological laboratories.

Journal de mycologie medicale·2020
Same author

[Cystoscopic treatment of an ureteral stricture following laparoscopic hysterectomy].

Progres en urologie : journal de l'Association francaise d'urologie et de la Societe francaise d'urologie·2020
Same author

Antifungal susceptibility testing practices in mycology laboratories in France, 2018.

Journal de mycologie medicale·2020
Same author

Borrelia crocidurae tick-borne relapsing fever upon return from Senegal.

Medecine et maladies infectieuses·2019
Same author

Candida auris in contemporary mycology labs: A few practical tricks to identify it reliably according to one recent French experience.

Journal de mycologie medicale·2018
Same author

Multiparametric evaluation of preoperative MRI in early stage breast cancer: prognostic impact of peri-tumoral fat.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2016

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cyclin-dependent kinases (Cdks) regulate essential eukaryotic cell cycle transitions.
  • D-type cyclins, cyclin E, and cyclin A are implicated in G1 and G1/S phase progression in mammalian cells.

Purpose of the Study:

  • To investigate if agents blocking the G1/S phase transition do so by regulating Cdk activities.
  • To identify the mechanisms of G1 arrest and Cdk inhibition.

Main Methods:

  • Inducing G1 arrest through various conditions: ionizing radiation, TGF-beta treatment, lovastatin treatment, and growth factor deprivation.
  • Analyzing the accumulation of Cdk inhibitor proteins.
  • Assessing the association of specific inhibitors (p21Waf1/Cip1, p27Kip1/p28Ick1) with different arrest conditions.

Related Experiment Videos

Main Results:

  • G1 arrest correlates with the accumulation of G1-specific Cdk inhibitors.
  • Cdk inhibition occurs through stoichiometric binding of Cdk inhibitor proteins.
  • p21Waf1/Cip1 accumulation is associated with DNA damage-induced arrest.
  • p27Kip1/p28Ick1 accumulation is observed under various antiproliferative conditions.

Conclusions:

  • Agents blocking the G1/S transition function by increasing Cdk inhibitor levels.
  • Cdk inhibitor proteins play a critical role in mediating cell cycle arrest at the G1/S phase.
  • Specific Cdk inhibitors are involved in distinct cellular responses to antiproliferative stimuli.