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The human myocardial two-dimensional gel protein database: update 1994
J M Corbett1, C H Wheeler, C S Baker
1Department of Cardiothoracic Surgery, National Heart and Lung Institute, Heart Science Centre, Middlesex, UK.
Electrophoresis
|November 1, 1994
Summary
This study presents an updated human heart protein database with 1388 characterized spots. Further analysis identified numerous proteins, aiding research into heart disease and transplantation.
Area of Science:
- Proteomics
- Cardiovascular Biology
- Biochemistry
Background:
- Two-dimensional electrophoresis (2-DE) is crucial for analyzing complex protein mixtures.
- A comprehensive human heart protein database is essential for understanding cardiac function and disease.
Purpose of the Study:
- To present an updated human heart protein two-dimensional electrophoresis (2-DE) database.
- To identify and characterize human heart proteins for further research.
Main Methods:
- Two-dimensional electrophoresis (2-DE) to separate proteins.
- Western immunoblotting and protein sequencing for protein identification.
- Database comparison for tentative protein assignments.
Main Results:
- The database contains 1388 characterized human heart protein spots (M(r) and pI).
- Western immunoblotting identified 49 protein spots.
- Protein sequencing identified 32 protein spots.
- Six additional proteins were tentatively assigned by database comparison.
Conclusions:
- The updated human heart protein 2-DE database is a valuable resource.
- This database facilitates studies on altered protein expression in diseased and transplanted hearts.
- Protein identification through sequencing and immunoblotting enhances the database's utility.