Related Experiment Video
Updated: Aug 8, 2026
Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
Published on: July 9, 2013
Immunohistochemical study of cellular fibronectin in preretinal membranes
1Department of Ophthalmology, School of Medicine, Fukuoka University, Japan.
Background:
The presence of fibronectin has been shown in preretinal membranes from eyes with proliferative vitreoretinopathy and proliferative diabetic retinopathy. It is not clear, however, which type of fibronectin (plasma or cellular) is present in preretinal membranes.
Methods:
A human cellular fibronectin antibody was used to immunohistochemically determine if cellular fibronectin only demonstrates presence or association with proliferation of preretinal membranes.
Results:
The results revealed a diffuse distribution of cellular fibronectin within membranes extracted from proliferative vitreoretinopathy and proliferative diabetic retinopathy patients, and a localized distribution of neovascular vessels in proliferative diabetic retinopathy membranes.
Conclusion:
Cellular fibronectin produced locally in preretinal membranes may contribute a local, cell-mediated process to cell proliferation or migration and the expression of cellular fibronectin by the cellular constituents of preretinal membranes is unique to this pathologic situation like proliferative diabetic retinopathy and proliferative vitreoretinopathy.
More Related Videos
10:46Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes
Published on: October 7, 2018
05:15Investigating Retinal Circuits and Molecular Localization by Pre-Embedding Immunoelectron Microscopy
Published on: July 12, 2024
