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Updated: Aug 9, 2026

Flow Cytometry-based Purification of S. cerevisiae Zygotes
Published on: September 21, 2012
[Chromosome fractionation in a saccharose density gradient for subsequent flow sorting]
Abstract:
The human chromosomes were obtained from lymphoblastoid cell line BOLD with normal karyotype, and separated on the basis of their size by velocity sedimentation at 190 g in sucrose gradient. Different chromosomal fractions were stained by applying fluorochromes with different DNA-base specificity: Hoechst 33258 and Olivomycine and were analysed using dual laser cell-sorter ATC-3000 (Brucker). Velocity sedimentation allowed to enrich certain fractions by individual chromosomes and thus speed up the sorting rate from 90 up to 200 chromosomes per second.
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