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Related Experiment Videos

Class II major histocompatibility complex-restricted T cell function in CD4-deficient mice

A Rahemtulla1, T M Kündig, A Narendran

  • 1Amgen Institute, Toronto, Ontario, Canada.

European Journal of Immunology
|September 1, 1994
PubMed
Summary

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CD4-deficient mice can mount antibody responses due to CD4-8-TcR alpha beta+ T cells, which provide "help" and are class II MHC-restricted. This suggests CD4 is not essential for helper T cell selection or function.

Area of Science:

  • Immunology
  • T cell biology
  • Adaptive immunity

Background:

  • CD4-deficient mice normally possess T and B cells, including CD4-8-TcR alpha beta+ T cells.
  • These mice paradoxically exhibit immunoglobulin isotype class switching from IgM to IgG.
  • The role of specific T cell subpopulations in this CD4-independent response remained unclear.

Purpose of the Study:

  • To identify the T cell population responsible for antibody "help" in CD4-deficient mice.
  • To determine the MHC restriction of these T cells.
  • To analyze the phenotype of thymocytes in CD4-deficient mice.

Main Methods:

  • In vivo T cell subpopulation depletion.
  • Antigen-specific proliferation assays.
  • Blocking studies using MHC-specific antibodies.

Related Experiment Videos

  • Phenotypic analysis of thymocytes.

    • Main Results:

    • CD4-8-TcR alpha beta+ T cells were identified as the source of "help" for antibody responses in CD4-deficient mice.
    • These T cells demonstrated class II major histocompatibility complex (MHC) restriction.
    • CD4-CD8- thymocytes in CD4-deficient mice exhibited a more mature phenotype compared to wild-type mice.

    Conclusions:

    • CD4-independent "help" for antibody responses is mediated by CD4-8-TcR alpha beta+ T cells.
    • These T cells are class II MHC-restricted.
    • CD4 is not essential for the positive selection or effector function of class II MHC-restricted helper T cells.