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Stimulation of insulin release by elevated pressure gradient
Endocrinology
|December 1, 1976
Summary
Elevated pressure increases insulin release from pancreatic beta cells by aiding granule translocation. This pressure effect is independent of glucose stimulation and DNP, suggesting a mechanical role in exocytosis.
Area of Science:
- Endocrinology
- Cell Biology
- Physiology
Background:
- Insulin release is crucial for glucose homeostasis.
- Intracellular mechanisms, including the microtubular-microfilamentous system, are involved in beta granule exocytosis.
Purpose of the Study:
- To investigate the effect of elevated pressure on insulin release rates.
- To explore the role of mechanical forces in beta granule exocytosis.
Main Methods:
- In vitro pancreatic preparations were incubated in low glucose media.
- Pressure gradients (0-45 mm Hg) were applied to the gas phase.
- Insulin release was measured under varying pressure conditions and in the presence of DNP.
Main Results:
- Increased pressure gradients directly correlated with higher insulin release rates.
- Pressure-induced insulin release persisted briefly after pressure release.
- Pressure-stimulated insulin release was unaffected by 1 mM DNP, unlike glucose-stimulated release.
Conclusions:
- Elevated pressure can stimulate insulin release, likely by providing motive force for beta granule translocation and exocytosis.
- This suggests a mechanical component in the secretory process of beta cells.
- Pressure may act on contractile structures within islet cells to facilitate granule extrusion.