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Bacterial transporters

P C Maloney1

  • 1Department of Physiology, Johns Hopkins Medical School, Baltimore, MD 21205.

Current Opinion in Cell Biology
|August 1, 1994
PubMed
Summary
This summary is machine-generated.

A new database of membrane transporter sequences aids evolutionary studies and functional homology detection. Structural analysis, including site-directed mutagenesis, may reveal transporter pathways at helical bundle interfaces.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Membrane transporters are crucial for cellular functions.
  • Understanding their evolutionary relationships and structural basis is key.
  • Existing research spans bacterial and eukaryotic systems.

Purpose of the Study:

  • To establish a comprehensive database of membrane transporter sequences.
  • To facilitate the study of evolutionary relationships among transporters.
  • To integrate findings from eukaryotic and prokaryotic model systems, including the major facilitator superfamily.

Main Methods:

  • Compilation of an extended database of bacterial membrane transporter sequences.
  • Comparative analysis of sequences to identify evolutionary relationships and functional homology.

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  • Structural analysis, including site-directed mutagenesis of a bacterial antiporter.
  • Preparation of two-dimensional crystals of the eukaryotic anion-exchange protein (band 3).

    • Main Results:

    • An extensive database of membrane transporter sequences is now available.
    • Evidence suggests functional homology even among evolutionarily distant carriers.
    • Site-directed mutagenesis provides insights into translocation pathways.
    • Progress in crystallizing membrane proteins like band 3 is noted.

    Conclusions:

    • The new database enables deeper study of membrane transporter evolution.
    • Structural and mutagenesis studies may soon elucidate the transporter pathway.
    • The proposed pathway lies at the interface of helical bundles.
    • This could lead to informed 3D model construction without crystallographic data.