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A rapid and efficient PCR-based method for synthesizing high-molecular-weight multimers of oligonucleotides
1Laboratory of Structural Biology and Molecular Medicine, UCLA School of Medicine 90024.
Biochemical and Biophysical Research Communications
|November 30, 1994
Abstract:
We have developed a simple PCR based approach for synthesizing high-molecular-weight DNA multimers of short oligonucleotide elements in yields sufficient for preparing DNA affinity columns or for using as probes to detect sequence-specific DNA binding proteins. The technique is rapid, efficient and produces multimers consisting of 30-100 repeating units.