[Isolation and characteristics of a continuous human splenic cell culture]

A continuous cell line of human spleen has been obtained. The methods of seeding large numbers of cells and prolonged cultivation of the culture played an important role in isolation of this cell line; both methods have contributed to the creation of conditions for gradual reconstruction of the cellular metabolism in the absence of which the cells wound not be able to get adapted to contiuous cultivation. As the authors failed to obtain a contiuous cell line from a single cell colony the method of "shaking" was applied. The culture was obtained only in case of a 1% PHA solution was added to the nutrient medium.