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fgfr-1 is required for embryonic growth and mesodermal patterning during mouse gastrulation
T P Yamaguchi1, K Harpal, M Henkemeyer
1Samuel Lunenfeld Research Institute (SLRI), Mount Sinai Hospital, Toronto, Ontario, Canada.
Genes & Development
|December 15, 1994
Summary
Fibroblast growth factor receptor 1 (FGFR-1) is crucial for mesodermal patterning in mouse embryos. Its absence leads to defects in somite formation and axial mesoderm expansion, impacting cell fate specification.
Area of Science:
- Developmental biology
- Molecular genetics
- Embryogenesis
Background:
- Fibroblast growth factors (FGFs) are known to play roles in mesoderm development in amphibians.
- The specific role of FGF signaling in mammalian embryogenesis requires further investigation.
Purpose of the Study:
- To genetically dissect the role of fibroblast growth factor signaling in mouse embryogenesis using fgfr-1 mutations.
- To understand the function of FGFR-1 in mesoderm generation, differentiation, and patterning.
Main Methods:
- Generation of fgfr-1 homozygous mutant mice.
- Analysis of embryonic development, gastrulation, and mesodermal patterning in mutant embryos.
Main Results:
- fgfr-1 signaling is not essential for gastrulation or initial mesoderm generation.
- Mutant embryos exhibit aberrant mesodermal patterning, including absent somites and expanded axial mesoderm.
- Nascent mesoderm differentiates into subtypes but lacks proper regional organization.
Conclusions:
- FGFR-1 signaling is critical for specifying mesodermal cell fates and regional patterning during gastrulation.
- The study highlights the essential role of FGFR-1 in establishing correct mesodermal structures during mammalian development.