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Detection of 13C labelled compounds by gas chromatography coupled to atomic emission detection--application to
M S Boukraa1, D Deruaz, A Bannier
1Laboratoire d'Etudes Analytiques et Cinétiques du Médicament-LEACM, Institute of Pharmaceutical and Biological Sciences, Lyon, France.
Abstract:
This paper illustrates the use of gas chromatography coupled to atomic emission detection (GC-AED) and of the stable isotope tracer 13C for the determination of drug metabolites. After administration of a parent drug labelled with 13C and extraction of the metabolites from the biological fluids, a 13C chromatographic profile is determined using the specific detection of the 13C atomic emission and subtraction of the 13C natural abundance. Thus, only the compounds which are metabolites with a 13C enrichment over the natural abundance are detected. [1,3,7 trimethyl-13C3]xanthine which is extensively metabolized by the liver is used as an example.