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Conformation polymorphisms and targeted marker development

B W Kirkpatrick1, G L Hart

  • 1Department of Meat and Animal Science, University of Wisconsin-Madison 53706.

Animal Genetics
|April 1, 1994
PubMed
Summary
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New genetic markers using DNA conformation polymorphisms were developed for cattle. While microsatellite markers showed higher heterozygosity, conformation polymorphisms are useful for linkage and comparative mapping in diverse cattle families.

Area of Science:

  • Genetics
  • Molecular Biology
  • Animal Breeding

Background:

  • Development of genetic markers is crucial for livestock improvement.
  • DNA conformation polymorphisms offer a potential alternative to existing marker systems.

Purpose of the Study:

  • To develop and evaluate novel genetic markers using DNA conformation polymorphisms in cattle.
  • To compare the utility of conformation polymorphisms with microsatellite markers for genetic mapping.

Main Methods:

  • Single-stranded and double-stranded DNA conformation polymorphisms were employed.
  • Nine DNA fragments from three bovine loci were analyzed.
  • Heterozygosity of parents from reference and resource families was compared between marker types.

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Main Results:

  • Eight of nine DNA fragments showed single-stranded conformation polymorphisms (SSCPs), with most exhibiting two alleles.
  • Only one fragment showed double-stranded conformation polymorphism (DSCP).
  • Microsatellite markers demonstrated significantly higher heterozygosity than conformation polymorphisms, particularly in resource families.

Conclusions:

  • DNA conformation polymorphisms are a valuable tool for targeted marker development in cattle.
  • Their utility is enhanced when studying genetically diverse families.
  • Conformation polymorphisms can aid in linkage and comparative map development.