Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Membrane ruffling and signal transduction

A J Ridley1

  • 1Ludwig Institute for Cancer Research, University College/Middlesex Hospital Branch, London, UK.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|May 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing the Quality of Models of the Ambient Solar Wind.

Space weather : the international journal of research & applications·2020
Same author

RhoA, RhoB and RhoC have different roles in cancer cell migration.

Journal of microscopy·2013
Same author

Analysis of Rho GTPase expression in T-ALL identifies RhoU as a target for Notch involved in T-ALL cell migration.

Oncogene·2012
Same author

A model of localised Rac1 activation in endothelial cells due to fluid flow.

Journal of theoretical biology·2011
Same author

Regulation of macrophage adhesion and migration by Rho GTP-binding proteins.

Journal of microscopy·2008
Same author

Differential effects of immunosuppressive drugs on T-cell motility.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2006
Same journal

AI in Genomics: From Variant Calling to Multi-Omics Integration.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Rethinking One Health: Microbial Foundations for Ecological Governance.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Biobanked Liver Organoids: A Roadmap for Precision Hepatology.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

The Temporal Architecture of Human Cells: Organelle Clocks and Distributed Circadian Time.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Opposing Activity at the Apical Surface: An Antagonistic Collaboration Between Crumbs and Myosin II Determines Organ Shape.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
Same journal

Hidden Fungal DNA Structures May Shape Sequencing Outcomes.

BioEssays : news and reviews in molecular, cellular and developmental biology·2026
See all related articles

Cell membrane ruffling, a key early response to extracellular signals, involves actin reorganization regulated by proteins like Rac. This process integrates various signaling molecules and cytoskeletal components.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Membrane ruffling is an early cellular response to extracellular factors, characterized by actin-rich protrusions.
  • Actin cytoskeleton reorganization is crucial for signal transduction pathways.
  • The small GTP-binding protein Rac is a key regulator of membrane ruffling.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying membrane ruffling.
  • To identify key signaling molecules and proteins involved in actin reorganization during membrane ruffling.
  • To understand the role of Rac and its regulators in this cellular process.

Main Methods:

  • Analysis of cellular responses to extracellular stimuli.
  • Investigation of actin dynamics and polymerization.

Related Experiment Videos

  • Biochemical assays to study protein-protein interactions and signaling pathways.
  • Localization studies of signaling molecules and actin-binding proteins.

    • Main Results:

    • Identified Rac as a central regulator of membrane ruffling and actin dynamics.
    • Demonstrated the involvement of multiple signaling molecules (Ras, Grb2, PI3K, PLA2) in the ruffling response.
    • Showed that proteins regulating Rac activity, such as Bcr, influence this pathway.
    • Observed the localization of actin-binding proteins to membrane ruffles, suggesting their role as targets.

    Conclusions:

    • Membrane ruffling is a complex process involving intricate crosstalk between signaling pathways and the actin cytoskeleton.
    • Rac and its regulators are critical components of the signal transduction machinery driving membrane ruffling.
    • Further research into actin-binding proteins in ruffles may reveal novel regulatory mechanisms.