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Immunophenotyping of normal lymphocytes

R E Peters1, S al-Ismail

  • 1Department of Haematology, Singleton Hospital, Swansea, Wales, UK.

Clinical and Laboratory Haematology
|March 1, 1994
PubMed
Summary
This summary is machine-generated.

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This study establishes reproducible normal reference values for peripheral blood mononuclear cell populations using flow cytometry. These findings provide essential benchmarks for clinical use in immunology and hematology.

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • Accurate enumeration of peripheral blood mononuclear cells is crucial for diagnosing and monitoring various health conditions.
  • Establishing reliable reference ranges for immune cell populations is essential for clinical interpretation.

Purpose of the Study:

  • To establish reproducible normal reference values for peripheral blood mononuclear cell populations.
  • To validate flow cytometric analysis for enumerating T cells, B cells, natural killer (NK) cells, and myeloid cells.
  • To assess the clinical utility of these reference values in both healthy individuals and patients with non-hematological disorders.

Main Methods:

  • Direct and indirect immunofluorescence labeling of peripheral blood mononuclear cells.
  • Flow cytometric analysis to define cell surface antigen expression.

Related Experiment Videos

  • Use of polyclonal and monoclonal antibodies for T cells, T-cell subsets, B cells, NK cells, and myeloid cells.
  • Comparison with established methods like whole blood lysis and E rosettes.

    • Main Results:

    • Normal values for absolute numbers and percentages of mononuclear cell populations were established with high reproducibility.
    • Flow cytometry results were comparable to other established immunological methods.
    • Immune cell recovery was generally complete, indicating the reliability of the method.
    • Results were consistent between normal controls and patients with non-hematological disorders.

    Conclusions:

    • The study successfully established clinically useful, reproducible reference values for peripheral blood mononuclear cell populations.
    • Flow cytometry is a reliable method for enumerating immune cell subsets.
    • These reference values serve as a crucial benchmark for lymphoid sub-populations in normal controls and can aid in clinical assessments.