L-glutamate-stimulated taurine release from rat cerebral cultured astrocytes

We characterized L-glutamate-stimulated taurine release from cultured astrocytes prepared from rat cerebrum. L-glutamate (0.5 mM) stimulated release of 3H-labeled and endogenous taurine, where the rate of release reached maximum in 40 min. L-glutamate increased astrocytic volume [3H-O-methyl-D-glucose (3H-OMG) space] with a similar time course to 3H-taurine release. Quisqualate, L-aspartate, DL-homocysteate, and L-cysteate increased both astrocytic 3H-OMG space and 3H-taurine release from cultured astrocytes, while kainate (1 mM) stimulated 3H-taurine release without affecting astrocytic volume. N-methyl-D-aspartate had no effect on 3H-taurine release and astrocytic volume. Treatment of astrocytes with dibutyryl cAMP reduced the effect of kainate on 3H-taurine release. L-glutamate-stimulated 3H-taurine release was attenuated by removal of extracellular Cl- and in hyperosmotic medium, which prevented L-glutamate-induced increase in 3H-OMG space of cultured astrocytes. These results indicate that L-glutamate stimulates taurine release from astrocytes through swelling-triggered mechanisms and that kainate causes the release through volume-independent mechanisms.