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Isolation of rotavirus strains from naturally infected piglets
1Veterinary Research Institute, Brno, Czech Republic.
Acta Virologica
|February 1, 1994
Abstract:
Twenty rotavirus strains were isolated in 1991-92 from 60 faecal samples collected from diarrhoeic piglets in the Czech and Slovak Republics. Three isolates were adapted to the growth in the cell line MA-104 and produced cytopathic effect. Rotavirus was demonstrated by immunofluorescence test, electron microscopy, polyacrylamide gel electrophoresis, immunoperoxidase test and ELISA.