Glucose potentiometric electrodes based on mediatorless bioelectrocatalysis. A new approach

Area of Science:

• Electrochemistry
• Biosensors
• Enzyme Catalysis

Background:

• Glucose analysis is crucial for diagnosing and managing diabetes.
• Existing glucose monitoring methods have limitations in sensitivity, stability, or cost.
• Enzyme-based electrochemical sensors offer a promising alternative for glucose detection.

Purpose of the Study:

• To develop a novel potentiometric method for glucose analysis.
• To utilize co-immobilized glucose oxidase and peroxidase on a composite carbon electrode.
• To establish a stable and sensitive biosensor for accurate glucose quantification.

Main Methods:

• Co-immobilization of glucose oxidase and peroxidase onto a composite carbon electrode surface.
• Potentiometric detection based on the electroreduction of hydrogen peroxide, a product of glucose oxidation.
• Linear regression analysis to determine the relationship between electrode potential shift and glucose concentration.

Main Results:

• The developed method demonstrated a linear calibration curve for glucose in the range of 0.025–2.0 mM.
• The biosensor exhibited high stability, allowing for at least 100 measurements without activity loss.
• Electrodes maintained stability for up to 90 days when stored at 277 K.

Conclusions:

• A robust and sensitive potentiometric glucose biosensor has been successfully developed.
• The mediatorless approach offers advantages in simplicity and cost-effectiveness.
• The sensor's stability and linear detection range make it suitable for practical glucose monitoring applications.