[Detection of numerical chromosome aberrations in human sperm nuclei using in situ hybridization from formarin-fixed...

It has been shown that molecular cytogenetics in interphase nuclei is applicable to human sperm nuclei from formarin-fixed clot sections utilizing in situ hybridization (ISH). Ejaculates from a normal volunteer were studied utilizing biotinylated DNA probes specific for the alpha satellite region for numerical aberrations of chromosome 17 and Y. With respect to the appearance of the ISH signal, optical concentration and time for the digestion enzyme has to be established essentially. This allows precise identification of numerical abnormalities of chromosome in sperm nuclei without disruption of the morphology. This technique can now be applied to the detection of chromosomal aneuploidy in human sperm from patients with male infertility.