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[Chromatomass-spectrometric determination of oxidized cholesterol derivatives in blood serum]
Abstract:
Content of several oxidized forms of cholesterol was studied in blood serum of patients with hypercholesterolemia using chromato-mass-spectrometry. The hydroxycholesterols detected were identified as 7-beta-hydroxycholesterol, 25-hydroxycholesterol, 7-ketocholesterol in the presence of standard substances added. Main patterns of gas chromatographic and mass spectrometric analyses of these substances are described.