Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA stretching on functionalized gold surfaces

R M Zimmermann1, E C Cox

  • 1Department of Molecular Biology, Princeton University, NJ 08544.

Nucleic Acids Research
|February 11, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The genome of the social amoeba Dictyostelium discoideum.

Nature·2005
Same author

lambda-Repressor oligomerization kinetics at high concentrations using fluorescence correlation spectroscopy in zero-mode waveguides.

Biophysical journal·2004
Same author

Insulating behavior of lambda-DNA on the micron scale.

Physical review letters·2002
Same author

Separation of 100-kilobase DNA molecules in 10 seconds.

Analytical chemistry·2002
Same author

Resetting wave forms in dictyostelium territories.

Physical review letters·2001
Same author

Near-field scanner for moving molecules.

Physical review letters·2001
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Researchers developed a novel method to anchor bacteriophage lambda DNA to gold surfaces. This DNA anchoring technique enables precise stretching and alignment of large DNA molecules using electric and flow fields.

Area of Science:

  • Biophysics
  • Molecular Biology
  • Nanotechnology

Background:

  • Precise manipulation of long DNA molecules is crucial for genomic studies.
  • Existing DNA anchoring methods face challenges in stability and control.

Purpose of the Study:

  • To develop a robust method for end-specific DNA anchoring to a gold surface.
  • To enable stretching and alignment of large DNA molecules using external fields.

Main Methods:

  • Utilized a multi-step binding strategy involving gold-biotin-streptavidin-biotin-DNA linkages.
  • Anchored bacteriophage lambda DNA to microfabricated gold lines.
  • Applied flow and electric fields to manipulate the anchored DNA.

Main Results:

Related Experiment Videos

  • Successfully anchored DNA molecules to gold surfaces with high specificity.
  • Demonstrated the ability to align and stretch DNA in response to applied fields.
  • Quantified the anchor's stability, showing resistance to forces of at least 11 pN.

    • Conclusions:

    • The developed Au-biotin-streptavidin-biotin-DNA linkage provides a stable anchor for DNA manipulation.
    • This method allows for controlled stretching and alignment of chromosome-sized DNA molecules.
    • The technique has significant potential for applications in genomics and molecular stretching assays.