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[Folacin determination in the blood by a fluorimetric method]
Voprosy Pitaniia
|January 1, 1975
Summary
A new fluorometric method significantly speeds up blood folacin determination from days to hours. This accurate assay offers a faster alternative to traditional microbiological methods for measuring folacin levels.
Area of Science:
- Biochemistry
- Analytical Chemistry
Background:
- Accurate determination of blood folacin is crucial for diagnosing deficiencies.
- Existing microbiological assays are time-consuming, requiring 4-5 days.
Purpose of the Study:
- To propose and validate a rapid fluorometric method for blood folacin determination.
- To compare the efficiency and accuracy of the fluorometric method against microbiological assays.
Main Methods:
- Enzymatic liberation of combined folacin forms from blood samples.
- Tyrosine quantification to correct for fluorescence changes during oxidation.
- Measurement of fluorescence before and after potassium permanganate oxidation.
Main Results:
- The fluorometric method reduces assay time from 4-5 days to 4-5 hours.
- Accuracy of the determination is maintained compared to microbiological methods.
- The method involves sequential steps including enzyme treatment, tyrosine correction, and oxidation.
Conclusions:
- The proposed fluorometric method is a significantly faster and accurate technique for blood folacin analysis.
- This method offers a practical alternative for clinical laboratories requiring rapid folacin level assessment.