Calcein release from temperature-sensitive liposome with or without stirring

¹Department of Hospital Pharmacy, Toyama Medical and Pharmaceutical University, Sugitani, Japan.

|January 1, 1994

Summary

Stirring temperature-sensitive liposomes (liposomes) reduces the temperature required for calcein release by 8 degrees Celsius. Mixed liposomes showed maximum release near 40-45°C, depending on stirring.

Area of Science:

Liposome research
Drug delivery systems
Physical chemistry

Background:

Temperature-sensitive liposomes are crucial for controlled drug release.
Understanding factors affecting liposome release kinetics is essential for optimizing therapeutic applications.
Liposome composition and environmental conditions significantly influence drug release profiles.

Purpose of the Study:

To investigate the effect of stirring on calcein release from dipalmitoylphosphatidylcholine (DPPC) liposomes.
To determine the optimal temperature for maximum calcein release from mixed DPPC-distearoylphosphatidylcholine (DSPC) liposomes under different stirring conditions.

Main Methods:

Utilized reverse phase evaporation vesicle (REV) technology to create liposomes.
Incorporated the fluorescent marker calcein into liposomes.
Measured calcein release rates at various temperatures with and without mechanical stirring.

Main Results:

Stirring decreased the temperature required for calcein release from DPPC REVs by approximately 8°C.
DPPC-DSPC mixed REVs exhibited maximum calcein release around 40°C with stirring.
Maximum release for mixed REVs without stirring was observed near 45°C.

Conclusions:

Stirring significantly alters the thermal transition and release characteristics of temperature-sensitive liposomes.
Liposome composition, specifically the inclusion of DSPC, can modulate the release temperature.
These findings provide valuable insights for designing advanced liposomal drug delivery systems with tunable release profiles.

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Calcein release from temperature-sensitive liposome with or without stirring

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Calcein release from temperature-sensitive liposome with or without stirring

Area of Science:

Background:

Purpose of the Study:

Main Methods:

Main Results:

Conclusions:

Related Concept Videos

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