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Caseicin, a bacteriocin from Lactobacillus casei

E Müller1, F Radler

  • 1Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität, Mainz, Germany.

Folia Microbiologica
|January 1, 1993
PubMed
Summary

The intracellular bacteriocin, caseicin 80, from Lactobacillus casei inhibits fructose incorporation into cells. This protein, likely encoded chromosomally, affects DNA and protein biosynthesis.

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Area of Science:

  • Microbiology
  • Bacteriocin Research
  • Lactobacillus casei

Background:

  • Bacteriocins are ribosomally synthesized antimicrobial peptides.
  • Lactobacillus casei produces various bioactive compounds, including bacteriocins.
  • Intracellular bacteriocins present unique challenges in purification and characterization.

Purpose of the Study:

  • To purify and characterize the intracellular bacteriocin caseicin 80 from Lactobacillus casei strain B80.
  • To investigate the effects of caseicin 80 on cellular biosynthesis pathways.

Main Methods:

  • Purification of caseicin 80 from cell extracts of Lactobacillus casei B80.
  • Determination of protein molecular mass using SDS-PAGE (implied).
  • Analysis of the impact of caseicin 80 on the incorporation of radiolabeled precursors (14C) for DNA, protein, and carbohydrate biosynthesis.

Main Results:

  • Caseicin 80 was purified as a thermolabile protein with an apparent molar mass of 42 kDa.
  • No plasmids were detected in the bacteriocinogenic strain, suggesting chromosomal encoding.
  • Caseicin 80 influenced DNA and protein biosynthesis, though likely not as a primary effect.
  • Caseicin 80 specifically inhibited the incorporation of fructose, but not glucose, into cellular material.

Conclusions:

  • Caseicin 80 is an intracellular bacteriocin produced by Lactobacillus casei B80, likely encoded on the chromosome.
  • The primary mechanism of action appears to involve the inhibition of fructose uptake or metabolism.
  • Further research is needed to elucidate the precise molecular targets and pathways affected by caseicin 80.

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