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Micellar electrokinetic chromatography of hydroxyproline and other secondary amino acids in biological samples with
K C Chan1, G M Janini, G M Muschik
1PRI/DynCorp, NCI Frederick Cancer Research and Development Center, MD 21702.
Journal of Chromatography
|December 22, 1993
Abstract:
Micellar electrokinetic chromatography (MEKC) with laser-induced fluorescence (LIF) was used for the rapid and sensitive detection of hydroxyproline in serum and hydrolyzed urine that were pre-column derivatized with 9-fluorenylmethyl chloroformate (FMOC). The application of the combined o-phthalaldehyde (OPA)/FMOC derivatization in MEKC for the selective detection of secondary amino acids in biological samples is investigated.