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Endotoxin adherence to and elution from two casting alloys
K L Knoernschild1, C A Lefebvre, G S Schuster
1Department of Oral Rehabilitation, School of Dentistry, Medical College of Georgia, Augusta 30912-1250.
The International Journal of Prosthodontics
|January 1, 1994
Summary
Bacterial lipopolysaccharide (LPS) adhered more to abraded Rexillium III dental alloy. Despite higher initial adherence and elution, over 99% of LPS remained on both tested alloys after 96 hours.
Area of Science:
- Biomaterials Science
- Dental Materials
- Microbiology
Background:
- Lipopolysaccharide (LPS) from Gram-negative bacteria is a key factor in implant-associated infections.
- The surface properties of dental casting alloys can influence bacterial adherence and biofilm formation.
- Understanding alloy-LPS interactions is crucial for developing infection-resistant dental prosthetics.
Purpose of the Study:
- To compare the relative affinity of bacterial lipopolysaccharide (LPS) for two dental casting alloys: Rexillium III and Harmony Hard.
- To investigate the impact of surface finish (airborne particle abraded vs. polished) on LPS adherence and elution.
- To quantify LPS retention on these alloys over time.
Main Methods:
- Discs of Rexillium III and Harmony Hard alloys with abraded and polished surfaces were incubated with Escherichia coli LPS.
- Control discs were incubated in LPS-free water.
- LPS adherence was measured initially, and elution was assessed over 96 hours by transferring discs to fresh media and testing eluates for LPS presence.
Main Results:
- Initial LPS adherence was significantly greater on abraded Rexillium III compared to other alloy surfaces (P < .05).
- Both polished and abraded Rexillium III showed significantly higher LPS elution rates (P = .0001).
- After 96 hours, over 99% of the initially adhered LPS remained on both Rexillium III and Harmony Hard discs, irrespective of surface treatment.
Conclusions:
- Abraded Rexillium III exhibits higher initial bacterial lipopolysaccharide adherence.
- While elution occurs, a substantial amount of LPS remains bound to both tested casting alloys.
- Surface treatment has a limited long-term impact on LPS retention, highlighting the need for strategies beyond surface modification to prevent bacterial adhesion on dental alloys.