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[Conjugative plasmid transfer in Staphylococcus aureus?]

V S Zueva, N V Klitsunova, O A Dmitrenko

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |January 1, 1994
    PubMed
    Summary
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    Bacteriophage presence in recipient cells significantly enhances plasmid transfer frequency, facilitating the transfer of both conjugative and nonconjugative plasmids. This finding aids in understanding microbial gene transfer mechanisms.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Electron Microscopy

    Background:

    • Plasmid transfer is crucial for bacterial adaptation and evolution.
    • The efficiency of plasmid transfer can be influenced by recipient cell characteristics.

    Purpose of the Study:

    • To investigate the role of recipient cell lysogeny in plasmid transfer efficiency.
    • To compare plasmid transfer rates of conjugative and nonconjugative plasmids.

    Main Methods:

    • Microbiological assays for plasmid transfer frequency.
    • Electron microscopy to visualize cell interactions during conjugation.
    • Utilized nitrocellulose filters and fluid culture systems.

    Main Results:

    • Low transfer frequency of conjugative plasmid pG873 and absence of transfer for nonconjugative plasmids rms7 and pE994 with nonlysogenic recipients.

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  • Recipient cells harboring prophage increased gentamicin-sensitive transconjugant detection 100-fold.
  • Prophage presence enabled the detection of rms7 and pE994 plasmid transfer.
  • Electron microscopy revealed cell fusion in lysogenic recipient crosses, not observed in nonlysogenic crosses.

    • Conclusions:

    • Recipient cell lysogeny significantly enhances plasmid transfer, including nonconjugative types.
    • Prophage presence may facilitate plasmid acquisition through mechanisms like cell fusion.
    • Understanding these mechanisms is vital for microbial genetics and biotechnology.